Molecular Formula | C11H16N2O6 |
Molar Mass | 272.25454 |
Water Solubility | Dissolves readily at 5 mg/ml in 0.05M potassium phosphate pH 7.5 to give a clear, pale-green solution. Soluble in distilled water or dilute buffer. |
Appearance | Bright yellow to yellow powder |
Color | green-yellow |
BRN | 3562140 |
Storage Condition | -70°C |
MDL | MFCD00131192 |
WGK Germany | 3 |
FLUKA BRAND F CODES | 10 |
TSCA | Yes |
Reference Show more | 1. Huang Xu, Zhang Lang, Hao Ji, Cheng Zhuo, Feng Tianhui, Shu Guangwen. Protective effect of Shi Chuanxiong polysaccharide on acute liver failure induced by lipopolysaccharide and D-galactosamine in mice [J]. Chinese Journal of Pharmacology and Toxicology, 2017,31(04):31-317. 2. [IF = 4.142] Li Dan et al."Sensitive comparative interaction assay for detecting xanthine in serum based on the inner filter effect of enzyme-catalyzed oxidation products to silicon nanoparticles." Anal Bioanal Chem. 2021 Feb;413(5):1405-1415 3. [IF = 4.142] Chi Zhongmei et al."A photonic crystal fiber-based flux sensor for simultaneous and sensitive detection of cytoplasmic acid agents." Anal Bioanal Chem. 2022 Feb;414(4):1641-1649 |
EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
Overview | alanine aminotransferase full name "glutamic Pyruvate Transaminase". Its function is to catalyze the reversible transfer of amino groups between glutamic acid and pyruvic acid to form α-ketoglutarate and alanine, and to use pyridoxal phosphate as coenzyme to maintain amino acid metabolism in cells. Alanine aminotransferase (GPT) is released into the blood when the liver, myocardium and other tissue cells are damaged. Clinically, the activity of GPT in blood is measured to understand the nature and degree of organ diseases. |
detection method | linear determination of alanine aminotransferase content in detection solution: add 0.2 ~ 3.4mL high-purity water to the centrifuge tube, 0.4 ~ 3.6mL copper nanocluster solution, 0.1mL Tiopronin solution, add 0.1mL different concentrations of 1 ~ 1000U/L alanine aminotransferase solution to the mixed solution, fully react for 1~15min, the fluorescence intensity before and after the addition of alanine aminotransferase was detected by a fluorescence Spectrophotometer. The copper nanocluster refers to a copper nanocluster based on glutathione as a stabilizer. The experimental results show that the concentration of alanine aminotransferase in the range of 1-1000U/L, the fluorescence intensity of copper nanoclusters and alanine aminotransferase concentration showed a linear relationship, the linear equation is DF = 116.90744 0.54101X(DF is the difference between the fluorescence intensity after adding alanine aminotransferase and the fluorescence intensity of copper nanoclusters after only adding Tiopronin, X is the concentration of alanine aminotransferase), the linear correlation coefficient was 0.992 and the detection limit was 0.61U/L. |